HIST1H3A/HIST2H3A/H3F3A (pThr11) 抗体
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- 抗原
- HIST1H3A/HIST2H3A/H3F3A
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抗原表位
- pThr11
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- 免疫原
- Peptide sequence around phosphorylation site of threonine 11(K-S-T(p)-G-G) derived from Human Histone H3.
- 亚型
- IgG
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- 应用备注
- WB:1:500-1:1000,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- 抗原
- HIST1H3A/HIST2H3A/H3F3A
- 背景
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Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Preuss U., Landsberg G., Scheidtmann K.H.
Nucleic Acids Res. 31:878-885(2003)
Shimada M., Niida H., Zineldeen D.H., Tagami H., Tanaka M., Saito H., Nakanishi M.Cell 132:221-232(2008)
Metzger E., Yin N., Wissmann M., Kunowska N., Fischer K., Friedrichs N., Patnaik D., Higgins J.M., Potier N., Scheidtmann K.H., Buettner R., Schule R.Nat. Cell Biol. 10:53-60(2008)Aliases: H3/a H3/m H3.3A, H3/c H3/o H3F3B, H3/d, H3/f, H3/h
- UniProt
- P68431, Q71DI3, P84243
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