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JNK 抗体 (pThr183, pTyr185)

Name: JNK 抗体 (pThr183, pTyr185)
Brand: antibodies-online
SKU: ABIN7138766
Availability: OutOfStock

This anti-JNK antibody is a 兔多克隆 antibody detecting JNK in WB, ELISA 和 IF. Suitable for 人.

Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody. (JNK 抗体 (pThr183, pTyr185))

Western blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody. (JNK 抗体 (pThr183, pTyr185))

Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody. (JNK 抗体 (pThr183, pTyr185))

3 images

产品编号 ABIN7138766

发货至: 中国

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Quick Overview for JNK 抗体 (pThr183, pTyr185) (ABIN7138766)

抗原

See all JNK (MAPK8) 抗体

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

适用

人

宿主

兔

克隆类型

多克隆

标记

This JNK antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF)

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pThr183, pTyr185

原理

Phospho-MAPK8/MAPK9/MAPK10 (Thr183/Tyr185) Antibody

交叉反应

小鼠, 大鼠

纯化方法

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

免疫原

Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from Human JNK1/JNK2/JNK3.

亚型

IgG
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 245-345) antibody
MAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183), (pTyr185) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 犬, 兔 WB, ELISA, IHC (p), FACS, ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 138-370) antibody
MAPK8 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody
MAPK8 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) antibody
MAPK8 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 2F11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 201-300) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 小鸡, 犬 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 145-419) antibody
MAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) antibody
MAPK8 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 318-427) antibody
MAPK8 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 151-250) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 小鸡, Pig WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

缓冲液

Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

别名

MAPK8

背景

Background:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity

Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
Ichijo, H. (1999) Oncogene 18, 6087-93.
Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
Aliases: Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46

UniProt

P45983, P45984, P53779

途径

MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins