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Recombinant RAF1 抗体 (pSer621)

This anti-RAF1 antibody is a 兔 单克隆 antibody detecting RAF1 in WB, ELISA 和 IF. Suitable for 人.
产品编号 ABIN7127739
发货至: 中国

Quick Overview for Recombinant RAF1 抗体 (pSer621) (ABIN7127739)

抗原

See all RAF1 抗体
RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This RAF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

克隆位点

1C2
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    pSer621

    交叉反应

    纯化方法

    Affinity-chromatography

    免疫原

    A synthesized peptide derived from human Phospho-RAF1 (S621)

    亚型

    IgG
  • 应用备注

    Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200,

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C,-80 °C

    储存方法

    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 抗原

    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

    别名

    RAF1

    背景

    Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

    Aliases: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF

    UniProt

    P04049

    途径

    MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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