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Recombinant JNK 抗体 (pThr183, pThr221)

Name: Recombinant JNK 抗体 (pThr183, pThr221)
Brand: antibodies-online
SKU: ABIN7127709
Availability: OutOfStock

This anti-JNK antibody is a 兔单克隆 antibody detecting JNK in WB, ELISA 和 IHC. Suitable for 人.

Western Blot Positive WB detected in 293 whole cell lysate(treated with EGF or not) All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1. (Recombinant JNK 抗体 (pThr183, pThr221))

Immunocytochemistry analysis of ABIN7127709 diluted at 1:165 and staining in Hela cells(treated with 100 ng/mL EGF for 4h) performed on a Leica BondTM system. (Recombinant JNK 抗体 (pThr183, pThr221))

ICC

2 images

产品编号 ABIN7127709

发货至: 中国

Quick Overview for Recombinant JNK 抗体 (pThr183, pThr221) (ABIN7127709)

抗原

See all JNK (MAPK8) 抗体

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

抗体类型

Recombinant Antibody

适用

人

宿主

兔

克隆类型

单克隆

标记

This JNK antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

克隆位点

1A9

Discover our top product MAPK8 Primary Antibody

抗原表位
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pThr183, pThr221

原理

Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody

纯化方法

Affinity-chromatography

免疫原

A synthesized peptide derived from human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)

亚型

IgG
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 245-345) antibody
MAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183), (pTyr185) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 犬, 兔 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 138-370) antibody
MAPK8 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) antibody
MAPK8 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 2F11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibody
MAPK8 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 201-300) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 小鸡, 犬 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 145-419) antibody
MAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) antibody
MAPK8 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 318-427) antibody
MAPK8 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 151-250) antibody
MAPK8 适用: 人, 小鼠, 大鼠, 小鸡, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB:1:500-1:5000, IHC:1:50-1:200

限制

仅限研究用
状态

Liquid

缓冲液

phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

别名

MAPK8

背景

Background: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).
Aliases: Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C

UniProt

P45983, P45984, P53779

途径

MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins