SPARC 抗体 (AA 1-200)
Quick Overview for SPARC 抗体 (AA 1-200) (ABIN7126968)
抗原
See all SPARC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-200
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特异性
- SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
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亚型
- IgG2b
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anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 101-200) antibody
SPARC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibodySPARC 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibodySPARC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19869 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibodySPARC 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibodySPARC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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别名
- SPARC
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背景
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Basement membrane protein 40, BM40, Cysteine rich protein, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine (SPARC),SPARC / Osteonectin
Cellular localisation: Secreted. Basement membrane. -
分子量
- 38kDa
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基因ID
- 6678, 111779, 708558
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UniProt
- P09486
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途径
- Autophagy
抗原
-
