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Recombinant LEF1 抗体 (AA 100-200)

This anti-LEF1 antibody is a 兔 单克隆 antibody detecting LEF1 in IHC (f). Suitable for 人.
产品编号 ABIN7126772
发货至: 中国

Quick Overview for Recombinant LEF1 抗体 (AA 100-200) (ABIN7126772)

抗原

See all LEF1 抗体
LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This LEF1 antibody is un-conjugated

应用范围

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Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • 抗原表位

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    AA 100-200

    特异性

    Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: Human tonsil and thymus.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

    别名

    LEF1

    背景

    Lymphoid enhancer-binding factor 1 (LEF1), T cell specific transcription factor 1 alpha (TCF1a), TCF1 alpha, TCF10, TCF7L3, Transcription factor T cell specific 1 alpha,LEF1 / TCF1 alpha (Transcription Factor)
    Cellular localisation: Nucleus.

    分子量

    44kDa

    基因ID

    51176, 726506

    UniProt

    Q9UJU2

    途径

    WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
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