APEX1 抗体
Quick Overview for APEX1 抗体 (ABIN7111688)
抗原
See all APEX1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Immunogen affinity purified
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
- APEX nuclease(multifunctional DNA repair enzyme) 1
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亚型
- IgG
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anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-242) antibodyAPEX1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H9 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 219-318) antibodyAPEX1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A2G7 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 2-318) antibodyAPEX1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) antibodyAPEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1518CT337-123-86-269-232 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (acLys6) antibodyAPEX1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (N-Term) antibodyAPEX1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500 - 1:2000, IHC: 1:50 - 1:100, IF: 1:50 - 1:100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
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有效期
- 12 months
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- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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别名
- APEX1
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背景
- Synonyms:APE, APE1, APEX, APX, HAP1, REF1 Background:Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic(AP) endodeoxyribonuclease in the DNA base excision repair(BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions(such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate(dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination(CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone(PTH) expression by binding to negative calcium response elements(nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant(CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
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分子量
- 36 kDa
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基因ID
- 328
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UniProt
- P27695
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途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
抗原
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