TRPC6 抗体 (Intracellular)
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- 抗原 See all TRPC6 抗体
- TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))
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抗原表位
- AA 24-38, Intracellular
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适用
- 小鼠
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宿主
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豚鼠
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克隆类型
- 多克隆
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标记
- This TRPC6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Guinea Pig Polyclonal Antibody to TRPC6 Channel
- 特异性
- Intracellular, N-terminus
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- Rat - identical, human - 15,16 amino acid residues identical
- 产品特性
- Guinea pig Anti-TRPC6 Antibody , raised in guinea pigs, is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPC6 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC6 Antibody (ABIN7043824, ABIN7043968 and ABIN7043969) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6
- 亚型
- IgG
- Top Product
- Discover our top product TRPC6 Primary Antibody
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anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 1-192) antibody
TRPC6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 500-600) antibodyTRPC6 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 249-265), (Middle Region) antibodyTRPC6 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB620777 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB591644 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB447436 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibodyVerified TRPC6 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 732-931) antibodyTRPC6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibodyTRPC6 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibodyTRPC6 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3F2-H10-F2 unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: (ABIN7237432)
Blocking Peptide: (ABIN7237432)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))
- 别名
- TRPC6 (TRPC6 产品)
- 背景
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Short transient receptor potential channel 6, Transient receptor protein 6, TRP6,Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH , endocrine factors as well as signaling molecules2.The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family form tetramers and could heterhomultimerize. They have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the transmembrane domain number 4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.The TRPC subfamily is further divided into the following: TRPC1/4/5, TRPC3/6/7 and TRPC21. Activation of phospholipase C (PLC) ultimately leads to the formation of diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP3) via hydrolysis of PIP2. The increase in concentration of these intracellular second messengers leads to the activation of non-selective Ca2+ channels and an IP3-induced release of Ca2+ from intracellular stores5. The intracellular Ca2+ store depletion in turn activates Ca2+ specific channels to allow replenish intracellular Ca2+ levels. TRPCs are thought to be activated upon intracellular store Ca2+ depletion, and may function in concert with the recently identified Orai channel5. The TRPC3/6/7 class produces similar currents upon activation.TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease6,7.
Alternative names: TRPC6, Short transient receptor potential channel 6, Transient receptor protein 6, TRP6 - 基因ID
- 22068
- NCBI登录号
- NM_004621
- UniProt
- Q61143
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