EPH Receptor A3 抗体 (Extracellular)
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- 抗原 See all EPH Receptor A3 (EPHA3) 抗体
- EPH Receptor A3 (EPHA3)
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抗原表位
- AA 488-500, Extracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EPH Receptor A3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to EphA3 Receptor
- 特异性
- Extracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat,mouse - identical
- 产品特性
- Anti-EphA3 (extracellular) Antibody (ABIN7043123, ABIN7044245 and ABIN7044246)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize EphA3 from human, rat, and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3
- 亚型
- IgG
- Top Product
- Discover our top product EPHA3 Primary Antibody
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anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibody
EPHA3 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 896-928) antibodyEPHA3 适用: 人, Hamster WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibodyEPHA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 896-928), (C-Term) antibodyEPHA3 适用: 人, Hamster WB, IHC (p) 宿主: 兔 Polyclonal RB1585 unconjugated
anti-EPH Receptor A3 (EPHA3) (C-Term) antibodyEPHA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 38-375) antibodyEPHA3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 751-983) antibodyEPHA3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 6C1B6 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 115-144) antibodyEPHA3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 401-500) antibodyEPHA3 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (C-Term) antibodyEPHA3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: BLP-ER013
Blocking Peptide: BLP-ER013
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- EPH Receptor A3 (EPHA3)
- 别名
- EPHA3 (EPHA3 产品)
- 背景
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Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4,Eph receptors are the largest family of receptor tyrosine kinases (RTKs). Based on sequence similarity and the type of ephrin ligands that they bind, they are classified into A and B sub-classes. EphA receptors bind ephrin-A ligand while EphB bind ephrin-B ligand.Eph receptors play an important role during embryonic development and are involved in several processes including cell adhesion, cell migration, and tissue boundary formation. Eph receptors are typically expressed at low levels in normal adult tissues1,2.EphA3 (Ephrin type-A receptor 3) structure includes a ligand-binding domain (LBD) at the N-terminus followed by a cysteine-rich region and two fibronectin III repeats. The receptor-binding domain of the ephrins is connected through a short linker segment to a GPI-anchor or a transmembrane segment3.EphA3 was first identified as a surface antigen on pre-B lymphoblastic leukemia cells. High EphA3 levels have been detected in a number of hematological cancers and solid tumors such as lung cancers, melanomas, gastric carcinoma, leukemia, in B and T cell malignancies and in glioblastoma multiforme. In addition, high levels of EphA3 are implicated in lymph node metastasis, advanced stages of colorectal cancer, and prostate cancer1,3,4.
Alternative names: EphA3, Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4 - 基因ID
- 2042
- NCBI登录号
- NM_005233
- UniProt
- P29320
- 途径
- RTK signaling, Regulation of Cell Size
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