ERK1 抗体 (AA 75-312)
Quick Overview for ERK1 抗体 (AA 75-312) (ABIN7433528)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 75-312
-
原理
- Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
-
特异性
- The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.
-
交叉反应
- 小鼠, Pig
-
纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
-
免疫原
- Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) corresdonding to Pro75~Thr312 with N-terminal His Tag
-
亚型
- IgG
-
-
-
-
应用备注
- Western blottin:g: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
-
说明
-
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
-
有效期
- 24 months
-
-
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
-
别名
- ERK1
-
背景
- HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
-
UniProt
- P27361
-
途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
-