PCNA 抗体 (AA 6-261)
Quick Overview for PCNA 抗体 (AA 6-261) (ABIN7443113)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 6-261
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原理
- Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
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特异性
- The antibody is a rabbit polyclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
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交叉反应
- 小鸡, Cow, 犬, 山羊, 豚鼠, 马, 小鼠, Pig, 大鼠, 绵羊
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Proliferating Cell Nuclear Antigen (PCNA) corresdonding to Leu6~Ser261 with N-terminal His and GST Tag
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亚型
- IgG
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应用备注
- Western blotting: 0.5-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.34 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin, Sodium azide
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注意事项
- This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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别名
- PCNA
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背景
- Cyclin
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
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