Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
SLCO1B1 antibody is human specific. At least four isoforms of SLCO1B1 are known to exist. This antibody is predicted to not cross-react with other SLCO1B proteins.
纯化方法
SLCO1B1 antibody is affinity chromatography purified via peptide column.
免疫原
SLCO1B1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLCO1B1. The immunogen is located within the last 50 amino acids of SLCO1B1.
Verified
SLCO1B1
适用: 人
ELISA, IF
宿主: 山羊
Polyclonal
unconjugated
应用备注
SLCO1B1 antibody can be used for detection of SLCO1B1 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SLCO1B1 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SLCO1B1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
SLCO1B1
(Solute Carrier Organic Anion Transporter Family, Member 1B1 (SLCO1B1))
SLCO1B1 is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and may play an important role in the clearance of bile acids and organic anions from the liver (1,2). It contains one Kazal-like domain and belongs to the organo-anion transporter family (2,3). SLCO1B1 is highly expressed in liver and is localized to the basolateral hepatocyte membrane. It is responsible for the hepatic uptake of the liver-specific hydroxymethylglutaryl-CoA reductase inhibitor in mouse, rat and human (3,4).