Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)
产品特性
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription. SMARCA2 / BRM antibody (mAb) (Clone 1H7A10) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
纯化方法
Protein G Chromatography
免疫原
This SMARCA2 / BRM antibody was raised against a recombinant protein corresponding to amino acids 48-214 of mouse BRM.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
SMARCA2
(SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))