Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins. Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb) (H2AZ) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Fission Yeast samples, but it is predicted that it will react with a wide range of sample types.
纯化方法
Affinity Purified
免疫原
This Pht1 / H2AZ acetyl antibody was raised against a peptide containing acetyl-lysine at positions 5, 7, 12 and 16 from the amino terminus of Pht1, the histone H2AZ variant in S. pombe.
亚型
IgG
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.