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CD14 抗体 (violetFluor™ 500)

This anti-CD14 antibody is a 小鼠 单克隆 antibody detecting CD14 in FACS. Suitable for 人.
产品编号 ABIN7278645
发货至: 中国

Quick Overview for CD14 抗体 (violetFluor™ 500) (ABIN7278645)

抗原

See all CD14 抗体
CD14

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD14 antibody is conjugated to violetFluor™ 500

应用范围

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Flow Cytometry (FACS)

克隆位点

61D3
  • 纯化方法

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

    亚型

    IgG1 kappa
  • 应用备注

    This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500, eFluor® 506 or Pacific Orange®.

    说明

    5ul (0.5ug)/test

    限制

    仅限研究用
  • 缓冲液

    10 mM NaH2PO4 , 150 mM NaCl, 0.09 % Sodium azide ,0.1 % gelatin, pH 7.2

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    2-8°C protected from light

    有效期

    12 months
  • 抗原

    CD14

    别名

    CD14

    背景

    The 61D3 antibody is specific for human CD14, a 53-55 kDa GPI-anchored glycoprotein. CD14 is highly expressed on monocytes and to a lesser extent on interfollicular macrophages, and some dendritic cells. Together with LPS-Binding Protein (LBP), CD14 binds to and mediates the innate immune response to bacterial lipopolysaccharide (LPS).

    基因ID

    929

    UniProt

    P08571

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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