Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4) (AA 36-41) 抗体
HPV-16 E1, E4
适用: Human Papillomavirus 16 (HPV-16)
IHC, StM
宿主: 小鼠
Monoclonal
HPV16 E1-E4
unconjugated
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- 抗原
- Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)
- 抗原表位
- AA 36-41
- 适用
- Human Papillomavirus 16 (HPV-16)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human HPV16 E1/E4 fragment (aa 36-41).
- 克隆位点
- HPV16 E1-E4
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: HPV-16 infected cells. Cervical tissue.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)
- 别名
- HPV16 E1/E4 (Human Papilloma Virus 16)
- 物质类
- Viral Protein
- 背景
- The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a 'high-risk' sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.
- 分子量
- 16kDa
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