G1/S-Specific B-Type Cyclin Cig2 (CIG2) 抗体
CIG2
适用: Schizosaccharomyces pombe
Coat, ELISA, WB
宿主: 小鼠
Monoclonal
3A11-5
unconjugated
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- 抗原 See all G1/S-Specific B-Type Cyclin Cig2 (CIG2) products
- G1/S-Specific B-Type Cyclin Cig2 (CIG2)
- 适用
- Schizosaccharomyces pombe
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Coating (Coat), ELISA, Western Blotting (WB)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Bacterial S. pombe Cig2 Purified protein.
- 克隆位点
- 3A11-5
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: Red blood cell lysate, Human colon and lung tissue.
Known Application: ELISA (For coating, order antibody without BSA),Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- G1/S-Specific B-Type Cyclin Cig2 (CIG2)
- 别名
- Cig2 (CIG2 产品)
- 别名
- G1/S-specific B-type cyclin Cig2 antibody, cig2 antibody
- 背景
- Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein.
- 分子量
- 50kDa
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