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TNF alpha 抗体 (AA 115-130)

Name: TNF alpha 抗体 (AA 115-130)
Brand: antibodies-online
SKU: ABIN6940754
Availability: OutOfStock

This anti-TNF alpha antibody is a 小鼠单克隆 antibody detecting TNF alpha in IHC, FACS, IF 和 StM. Suitable for 人, 小鼠, 大鼠, 兔, 犬, Cat 和斑马鱼.

Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Mouse Monoclonal Antibody (4C6-H8). (TNF alpha 抗体 (AA 115-130))

IHC

Immunofluorescence staining of paraformaldehyde-fixed HePG2 cells with TNF alpha Mouse Monoclonal Antibody (4C6-H8) followed by goat anti-Mouse IgG-CF488 (Green). (TNF alpha 抗体 (AA 115-130))

2 images

产品编号 ABIN6940754

发货至: 中国

Quick Overview for TNF alpha 抗体 (AA 115-130) (ABIN6940754)

抗原

See all TNF alpha 抗体

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

适用

人, 小鼠, 大鼠, 兔, 犬, Cat, 斑马鱼

宿主

小鼠

克隆类型

单克隆

标记

This TNF alpha antibody is un-conjugated

应用范围

Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

克隆位点

4C6-H8

Discover our top product TNF alpha Primary Antibody

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AA 115-130

免疫原

A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF- , conjugated to thyroglobulin

亚型

IgM kappa
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibody
TNF alpha 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)
TNF alpha 适用: 人, Pig, Non-Human Primate ICFC 宿主: 小鼠 Monoclonal MAb11 FITC

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibody
TNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

应用备注

Positive Control: HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF

背景

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

分子量

17kDa

基因ID

7124

UniProt

P01375

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome