BCL2L1 抗体 (AA 3-14)
Quick Overview for BCL2L1 抗体 (AA 3-14) (ABIN6940510)
抗原
See all BCL2L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 3-14
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特异性
- Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
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纯化方法
- Purified by Protein A/G
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免疫原
- A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein
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亚型
- IgG2a kappa
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应用备注
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Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- BCL2L1 (BCL2-Like 1 (BCL2L1))
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别名
- BCL2L1
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分子量
- 27kDa
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基因ID
- 598
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UniProt
- Q07817
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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