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BAX 抗体 (AA 3-16)

The 小鼠单克隆 anti-BAX antibody is suitable to detect BAX in samples from 人和猴. It has been validated for WB, IHC, IF, FACS 和 StM.

IHC

SDS-PAGE Analysis Purified Bax Mouse Monoclonal Antibody (2D2). (BAX 抗体 (AA 3-16))

SDS

Confocal Immunofluorescence of Human Jurkat cells A. (BAX 抗体 (AA 3-16))

Flow Cytometric Analysis of human Jurkat cells using Bax Mouse Monoclonal Antibody (2D2) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red). (BAX 抗体 (AA 3-16))

FACS

4 images

产品编号 ABIN6940472

发货至: 中国

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中国
北京 101111

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Quick Overview for BAX 抗体 (AA 3-16) (ABIN6940472)

抗原

See all BAX 抗体

BAX (BCL2-Associated X Protein (BAX))

适用

人, 猴

宿主

小鼠

克隆类型

单克隆

标记

This BAX antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

2D2

Discover our top product BAX Primary Antibody

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AA 3-16

特异性

Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

无交叉反应

小鼠, 大鼠

纯化方法

Purified by Protein A/G

免疫原

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

亚型

IgG1 kappa
anti-BCL2-Associated X Protein (BAX) (AA 84-175) antibody
BAX 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 小鸡, 斑马鱼 WB, ELISA, FACS, IP, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated X Protein (BAX) (AA 1-100) antibody
BAX 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated X Protein (BAX) (N-Term) antibody
BAX 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 兔, 小鸡, 犬, 绵羊, Hamster, 马, 豚鼠, Avian, Cat, 驴, 山羊 WB, IF 宿主: 山羊 Polyclonal unconjugated

anti-BCL2-Associated X Protein (BAX) (AA 1-158) antibody
BAX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-BCL2-Associated X Protein (BAX) antibody
KD Validated BAX 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 25GB450 unconjugated Recombinant Antibody

anti-BCL2-Associated X Protein (BAX) (AA 1-192) antibody
BAX 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F5-1B7 unconjugated

anti-BCL2-Associated X Protein (BAX) antibody
BAX 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2D8H2 unconjugated

anti-BCL2-Associated X Protein (BAX) (AA 13-160) antibody
BAX 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 4H9B11 unconjugated

anti-BCL2-Associated X Protein (BAX) antibody
BAX 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G6B2 unconjugated

anti-BCL2-Associated X Protein (BAX) (AA 19-33), (N-Term) antibody
BAX 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

BAX (BCL2-Associated X Protein (BAX))

别名

BAX

分子量

21kDa

基因ID

581

UniProt

Q07812

途径

p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response