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alpha Fetoprotein 抗体

AFP 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal MBS-12 unconjugated
产品编号 ABIN6934709
发货至: 中国
  • 抗原 See all alpha Fetoprotein (AFP) 抗体
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This alpha Fetoprotein antibody is un-conjugated
    应用范围
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    Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    特异性
    It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.
    纯化方法
    Purified by Protein A/G
    免疫原
    Human Alpha feto protein
    克隆位点
    MBS-12
    亚型
    IgG1 kappa
    Top Product
    Discover our top product AFP Primary Antibody
  • 应用备注

    Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    别名
    AFP (AFP 产品)
    别名
    FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
    分子量
    70kDa
    基因ID
    174
    UniProt
    P02771
    途径
    C21-Steroid Hormone Metabolic Process
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