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CD21 抗体 (AA 142-240)

This anti-CD21 antibody is a 小鼠 单克隆 antibody detecting CD21 in FACS, IHC, WB, IF, ELISA, StM 和 Coat. Suitable for 人.
产品编号 ABIN6939184
发货至: 中国

Quick Overview for CD21 抗体 (AA 142-240) (ABIN6939184)

抗原

See all CD21 (CR2) 抗体
CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD21 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)

克隆位点

CR2-2754
  • 抗原表位

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    AA 142-240

    特异性

    Recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: MOLT4, Raji or WEHI-231 cells. Tonsil.

    Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

    别名

    CR2

    分子量

    140kDa

    基因ID

    1380

    UniProt

    P20023

    途径

    Complement System
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