COL7A1 抗体
-
- 抗原 See all COL7A1 抗体
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- 适用
- 人, Cow, 山羊, 豚鼠, 马, 绵羊
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This COL7A1 antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant full-length human Collagen VII protein
- 克隆位点
- LH7-2
- 亚型
- IgG2a kappa
-
anti-Collagen, Type VII, alpha 1 (COL7A1) antibody
COL7A1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VII, alpha 1 (COL7A1) (C-Term) antibodyCOL7A1 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VII, alpha 1 (COL7A1) (Internal Region) antibodyVerified COL7A1 适用: 大鼠 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VII, alpha 1 (COL7A1) (AA 1841-1890) antibodyCOL7A1 适用: 人, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- 别名
- COL7A1 (COL7A1 产品)
- 别名
- COL7A1 antibody, EBD1 antibody, EBDCT antibody, EBR1 antibody, AW209154 antibody, collagen type VII alpha 1 chain antibody, collagen, type VII, alpha 1 antibody, COL7A1 antibody, col7a1 antibody, Col7a1 antibody
- 分子量
- 290kDa
- 基因ID
- 1294
- UniProt
- Q02388
-
