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SPTA1 抗体 (AA 356-475)

This anti-SPTA1 antibody is a 小鼠 单克隆 antibody detecting SPTA1 in ELISA, Coat 和 IHC (f). Suitable for 人.
产品编号 ABIN6938436
发货至: 中国

Quick Overview for SPTA1 抗体 (AA 356-475) (ABIN6938436)

抗原

See all SPTA1 抗体
SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

适用

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宿主

  • 20
  • 8
小鼠

克隆类型

  • 14
  • 14
单克隆

标记

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This SPTA1 antibody is un-conjugated

应用范围

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ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

SPTA1-1810
  • 抗原表位

    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 356-475

    特异性

    Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)

    亚型

    IgG2c kappa
  • 应用备注

    Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Jurkat cells. Placenta or Tonsil.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

    别名

    SPTA1

    背景

    Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1,Spectrin Alpha 1 (Erythrocyte Marker)
    Cellular localisation: Cytoplasmic and Cell Surface

    分子量

    240kDa

    基因ID

    6708, 119825

    UniProt

    P02549

    途径

    Regulation of Actin Filament Polymerization
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