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Recombinant SPTA1 抗体 (AA 356-475)

The 兔 单克隆 anti-SPTA1 antibody is suitable to detect SPTA1 in samples from 人. It has been validated for WB, IHC 和 ELISA.
产品编号 ABIN6940646
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant SPTA1 抗体 (AA 356-475) (ABIN6940646)

抗原

See all SPTA1 抗体
SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

抗体类型

Recombinant Antibody

适用

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宿主

  • 45
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克隆类型

  • 68
  • 14
单克隆

标记

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This SPTA1 antibody is un-conjugated

应用范围

  • 62
  • 59
  • 36
  • 14
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

克隆位点

SPTA1-2939R
  • 抗原表位

    • 43
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 356-475

    原理

    Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody

    特异性

    Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)

    亚型

    IgG, kappa
  • 应用备注

    Positive Control: K562, HeLa, Jurkat cells. Placenta or Tonsil.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

    别名

    SPTA1

    背景

    Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1

    HeLa, Jurkat cells. Placenta or Tonsil., K562, Human Kidney, Human Lung

    分子量

    240kDa

    基因ID

    6708, 119825

    UniProt

    P02549

    途径

    Regulation of Actin Filament Polymerization
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