SPI1 抗体 (AA 16-170)
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- 抗原 See all SPI1 抗体
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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抗原表位
- AA 16-170
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SPI1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
- 克隆位点
- PU1-2146
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product SPI1 Primary Antibody
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anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 16-170) antibody
SPI1 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal PU1-2146 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 124-271) antibodySPI1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5C7 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-120) antibodySPI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G1 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (Internal Region) antibodyVerified SPI1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-122) antibodySPI1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246) antibodySPI1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-264) antibodySPI1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 20-70) antibodySPI1 适用: 人, 小鼠, 犬, Cow, 马, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 18-196) antibodySPI1 适用: 人, 小鼠, 大鼠 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246), (C-Term) antibodySPI1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- 别名
- SPI1 (SPI1 产品)
- 别名
- OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody
- 背景
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Transcription Factor spi1, 31 kDa Transforming Protein, Hematopoietic Transcription Factor PU.1, SFPI1, SPI1, SPIA, Spleen focus forming virus (SFFV) proviral integration oncogene spi1,PU.1 (SPI-1) (B-Cell Marker)
Cellular localisation: Nuclear - 分子量
- 40kDa
- 基因ID
- 6688, 502511
- UniProt
- P17947
- 途径
- Stem Cell Maintenance
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