CD73 抗体
Quick Overview for CD73 抗体 (ABIN6938090)
抗原
See all CD73 (NT5E) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human NT5E protein
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亚型
- IgG1 kappa
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, IHC, FACS, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibodyNT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells in 0.1ml),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: A431, U87MG or PC-3 cells. Tonsil, Prostate or Lung Carcinoma
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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别名
- NT5E
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背景
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5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase,CD73 (Immuno-Oncology Target)
Cellular localisation: Cell surface -
分子量
- 71kDa
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基因ID
- 4907, 153952
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UniProt
- P21589
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途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
抗原
-
