Myc Tag 抗体 (AA 408-439)
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- 抗原 See all Myc Tag products
- Myc Tag
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抗原表位
- AA 408-439
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Myc Tag antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
- 无交叉反应
- 小鸡, 小鼠, 大鼠
- 交叉反应 （详细）
- Human. Does not react with Mouse, Rat or Chicken.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
- 克隆位点
- 9E10-3
- 亚型
- IgG1 kappa
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anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag antibodyWB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 408-439) antibody适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 cells. Cervical Carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Myc Tag
- Abstract
- Myc Tag 产品
- 物质类
- Tag
- 背景
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Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog,c-Myc Oncoprotein
Cellular localisation: Nuclear - 分子量
- 62-64kDa
- 基因ID
- 4609, 202453
- UniProt
- P01106
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