Keratin 77 抗体
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- 抗原 See all Keratin 77 (KRT77) 抗体
- Keratin 77 (KRT77)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 77 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- 交叉反应 (详细)
- Human. Rat. Shows broad species reactivity.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant human KRT77 protein
- 克隆位点
- KRTL-1077
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT77 Primary Antibody
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- 应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Skin, Squamous cell carcinoma (SCC).
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Keratin 77 (KRT77)
- 别名
- KRT77 (KRT77 产品)
- 别名
- K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
- 背景
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K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
- 基因ID
- 374454, 334989
- UniProt
- Q7Z794
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