KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18 抗体
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- 抗原
- KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- 交叉反应 （详细）
- Human. Cow. Rat. Mouse. Guinea Pig. Frog. Goat. Marmoset. Pig.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Keratin-enriched preparation from cultured human A431
- 克隆位点
- C11
- 亚型
- IgG1 kappa
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anti-KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18 antibody
适用: 人, Marmoset, 小鼠, Pig, Cow, 大鼠, Frog, 山羊, 豚鼠 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal C11 unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or A431 cells. Skin or colon carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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