HPV18 抗体
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- 抗原 See all HPV18 (HPV-18) products
- HPV18 (HPV-18) (Human Papilloma Virus Type 18 (HPV-18))
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适用
- Human Papillomavirus 18 (HPV-18)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HPV18 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- HPV18 E6-β-galactosidase fusion protein
- 克隆位点
- HPV18-1297
- 亚型
- IgG1 kappa
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anti-Human Papilloma Virus Type 18 (HPV-18) (AA 1-83), (N-Term) antibody
HPV-18 适用: 人 WB, EIA, IF 宿主: 小鼠 Monoclonal 2-00E-007 unconjugated
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- 应用备注
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Positive Control: HPV-18 infected cells. Cervical tissue.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- HPV18 (HPV-18) (Human Papilloma Virus Type 18 (HPV-18))
- 别名
- HPV-18 (Human Papilloma Virus 18) (HPV-18 产品)
- 物质类
- Viral Protein
- 背景
- Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90 % of cervical cancers contain HPV DNA of the high risk types. Mutational analysis has shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.
- 分子量
- 16kDa
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