Cytochrome C 抗体 (AA 1-80)
-
- 抗原 See all Cytochrome C (CYCS) 抗体
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
-
抗原表位
- AA 1-80
-
适用
- Pigeon
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Cytochrome C antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- 交叉反应 （详细）
- Human, Mouse, Rat, Horse, Dog, Pigeon, Frog and Drosophila.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
- 克隆位点
- 7H8-2C12
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CYCS Primary Antibody
-
anti-Cytochrome C, Somatic (CYCS) (AA 51-105) antibody
CYCS 适用: 人, 大鼠, 小鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 13-99) antibodyCYCS 适用: 大鼠, 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1268-1341) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5E6B11 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) antibodyCYCS 适用: 人, 大鼠, 小鼠, 马, 犬, Pigeon, Drosophila melanogaster, Frog IHC, StM 宿主: 兔 Monoclonal CYCS-3128R unconjugated Recombinant Antibody
-
- 应用备注
-
Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
- 别名
- CYCS (CYCS 产品)
- 别名
- CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody
- 背景
-
CYC, CYCS, HCS, THC4,Cytochrome C (Mitochondrial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 15kDa
- 基因ID
- 54205, 437060
- UniProt
- P99999
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
-
