BCL2L1 抗体 (AA 3-14)
Quick Overview for BCL2L1 抗体 (AA 3-14) (ABIN6936485)
抗原
See all BCL2L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 3-14
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特异性
- Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
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交叉反应 (详细)
- Human. Mouse. Rat. Pig.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.
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亚型
- IgG2a kappa
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应用备注
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Known_Application: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- BCL2L1 (BCL2-Like 1 (BCL2L1))
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别名
- BCL2L1
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背景
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Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52),Bcl-X (Apoptosis Marker)
Cellular localisation: Cytoplasmic and cell/nuclear membrane -
分子量
- 27kDa
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基因ID
- 598, 516966
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UniProt
- Q07817
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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