BAX 抗体 (AA 3-16)
Quick Overview for BAX 抗体 (AA 3-16) (ABIN6936465)
抗原
See all BAX 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 3-16
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特异性
- Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
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无交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Human. Monkey. Does not react with Mouse or Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
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亚型
- IgG1 kappa
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anti-BCL2-Associated X Protein (BAX) (AA 84-175) antibody
BAX 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 小鸡, 斑马鱼 WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-100) antibodyBAX 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 兔, 犬, 小鸡, 绵羊, 马, 豚鼠, 山羊, Hamster, Avian, Cat, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-158) antibodyBAX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-192) antibodyBAX 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F5-1B7 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 13-160) antibodyBAX 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 4H9B11 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G6B2 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2D8H2 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 41-76) antibodyBAX 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB0952 unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, Pig, Cow, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- BAX (BCL2-Associated X Protein (BAX))
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别名
- BAX
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背景
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Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4,Bax (Apoptosis Marker)
Cellular localisation: Cytoplasmic -
分子量
- 21kDa
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基因ID
- 581, 624291
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UniProt
- Q07812
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途径
- p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
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