-
- 抗原
- CK8 & CK18
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
- 交叉反应 （详细）
- Human.
- 免疫原
- Cytoskeleton preparation from human breast cancer MCF-7 cells
- 克隆位点
- 5D3
- 亚型
- IgG1 kappa
-
anti-CK8 & CK18 antibody
适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal 5D3 unconjugated
-
- 应用备注
-
Known_Application: Flow Cytometry (2-5 µL/million cells), Immunofluorescence (1:100-1:200), Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
- 限制
- 仅限研究用
-
- 缓冲液
- Tissue culture supernatant with 0.05% Azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- CK8 & CK18
- 背景
-
CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 8/18
Cellular localisation: Cytoplasmic - 分子量
- 52.5kDa (CK8), 45kDa (CK18)
- 基因ID
- 3856, 3875, 533782
- UniProt
- P05787, P05783
-
You are here:
