PCNA 抗体 (AA 201-261)
Quick Overview for PCNA 抗体 (AA 201-261) (ABIN676598)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
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    - 
                                            抗原表位
- AA 201-261
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                                            交叉反应
- 小鸡, 人, 小鼠, 大鼠
- 
                                            预测反应
- Dog,Cow,Rabbit
- 
                                            纯化方法
- Purified by Protein A.
- 
                                            免疫原
- KLH conjugated synthetic peptide derived from human PCNA
- 
                                            亚型
- IgG
 
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                                            应用备注
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                        WB 1:300-5000
 ELISA 1:500-1000
 FCM 1:20-100
 IHC-P 1:200-400
 IHC-F 1:100-500
 IF(IHC-P) 1:50-200
 IF(IHC-F) 1:50-200
 IF(ICC) 1:50-200
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                                            限制
- 仅限研究用
 
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    - 
                                            状态
- Liquid
- 
                                            浓度
- 1 μg/μL
- 
                                            缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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                                            储存液
- ProClin
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                                            注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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                                            储存条件
- 4 °C,-20 °C
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                                            储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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                                            有效期
- 12 months
 
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    - PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- 
                                            别名
- PCNA
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                                            背景
- 
                        Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. 
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                                            基因ID
- 5111
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                                            UniProt
- P12004
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                                            途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
 抗原
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