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PCNA 抗体 (AA 151-261)

Name: PCNA 抗体 (AA 151-261)
SKU: ABIN676583
Availability: OutOfStock

This anti-PCNA antibody is a 兔多克隆 antibody detecting PCNA in WB, ELISA, IHC (p), IF (p), IF (cc) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 6+ publications.

Mouse spleen lysates probed with PCNA Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (PCNA 抗体 (AA 151-261))

Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PCNA 抗体 (AA 151-261))

IHC

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (ABIN676583) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PCNA 抗体 (AA 151-261))

IHC

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti- PCNA-Proliferation Marker Polyclonal Antibody, Unconjugated (ABIN676583) at 1:100 followed by conjugation to the secondary antibody and DAB staining (PCNA 抗体 (AA 151-261))

IHC

IHC (p)

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (PCNA 抗体 (AA 151-261))

IHC (p)

6 images

(6 references)

产品编号 ABIN676583

发货至: 中国

Quick Overview for PCNA 抗体 (AA 151-261) (ABIN676583)

抗原

See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PCNA antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 151-261

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PCNA

亚型

IgG
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H10 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibody
PCNA 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17486 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhao, Han, Hong, Sun: "Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3079-3086, (2018) (PubMed).

Zhou, Zhang, Gao, Tan, Zheng, Zhao, Zhang: "Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 473-484, (2018) (PubMed).

Zhao, Feng, Zhang, Kou, Li, Liu, Zhang, Cui, Chu, Shen, Min: "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." in: Theriogenology, Vol. 89, pp. 280-288, (2017) (PubMed).

Ji, Liu, Zhao, Guo, Xia, Lu, Yin, Xu: "Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7391-7397, (2017) (PubMed).

Liang, Peng, Li, Yang, Xie, Li, Du, Zhang: "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." in: Oncotarget, Vol. 6, Issue 2, pp. 1020-30, (2015) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA

背景

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

基因ID

5111

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy