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YAP1 抗体 (AA 351-400)

This anti-YAP1 antibody is a 兔 多克隆 antibody detecting YAP1 in WB, IHC 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 斑马鱼, 犬, 猴, 绵羊, 小鸡, Cow, 马, Pig, 兔, 非洲爪蟾 和 Bat.
产品编号 ABIN462247
发货至: 中国

Quick Overview for YAP1 抗体 (AA 351-400) (ABIN462247)

抗原

See all YAP1 抗体
YAP1 (Yes-Associated Protein 1 (YAP1))

适用

人, 小鼠, 大鼠, 斑马鱼, 犬, 猴, 绵羊, 小鸡, Cow, 马, Pig, 兔, 非洲爪蟾, Bat

宿主

  • 130
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  • 1
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  • 1

克隆类型

  • 112
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  • 2
多克隆

标记

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This YAP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 28
    • 15
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    • 1
    • 1
    • 1
    AA 351-400

    品牌

    IHC-plus™

    特异性

    Human YAP1

    预测反应

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa351-400 of human YAP1 (P46937, NP_001123617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled Water.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    YAP1 (Yes-Associated Protein 1 (YAP1))

    别名

    YAP / YAP1

    背景

    Name/Gene ID: YAP1

    Synonyms: YAP1, 65 kDa Yes-associated protein, YAP, Yes-associated protein beta, Yes-associated protein delta, Yorkie homolog, YAP2, Yes-associated protein 1, Yes-associated protein 2, YKI, YAP65

    基因ID

    10413

    NCBI登录号

    NP_001123617

    UniProt

    P46937

    途径

    MAPK Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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