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NR1I3 抗体 (AA 180-229)

NR1I3 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Cow, Pig, 马, 兔, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN462245
发货至: 中国
  • 抗原 See all NR1I3 抗体
    NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
    抗原表位
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 180-229
    适用
    人, 小鼠, 大鼠, 犬, 豚鼠, Cow, Pig, 马, 兔, 猴, Bat
    宿主
    • 56
    • 5
    克隆类型
    • 60
    • 3
    多克隆
    标记
    • 39
    • 6
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NR1I3 antibody is un-conjugated
    应用范围
    • 49
    • 26
    • 16
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human NR1I3
    预测反应
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Bovine, Guinea pig (92%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%), Zebrafish (85%), Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product NR1I3 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
    别名
    NR1I3 / CAR (NR1I3 产品)
    别名
    AA209988 antibody, AI551208 antibody, CAR antibody, CAR-beta antibody, Care2 antibody, ESTM32 antibody, MB67 antibody, CAR1 antibody, CXR antibody, nuclear receptor subfamily 1 group I member 3 antibody, nuclear receptor subfamily 1, group I, member 3 antibody, NR1I3 antibody, Nr1i3 antibody
    背景
    Name/Gene ID: NR1I3
    Subfamily: NR1 Thyroid hormone-like
    Family: NHR

    Synonyms: NR1I3, Car-beta, CAR1, Constitutive active receptor, Constitutive active response, Orphan nuclear receptor MB67, MB67, CAR
    基因ID
    9970
    NCBI登录号
    NP_001070939
    UniProt
    Q14994
    途径
    Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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