His Tag 抗体 (Cy3)
(3 references)
Quick Overview for His Tag 抗体 (Cy3) (ABIN6720721)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-304-382
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Supplier
- Rockland
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原理
- 6X His Tag CY3 Conjugated Antibody
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交叉反应 （详细）
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.
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纯化方法
- 6X HIS Epitope Tag CY3 conjugated antibody was purified from concentrated tissue culture supernate by Protein A chromatography is directed against the 6X His motif and is useful in determining its presence in various assays.
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免疫原
- HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
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亚型
- IgG1 kappa
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Labeling Ratio
- 8.3
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (DyLight 800)WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
anti-His Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
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应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
ELISA_Dilution: User Optimized
Flow_Cytometry_Dilution: 1:500 - 1:2,500
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Western_Blot_Dilution: User Optimized
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说明
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Suggested Applications: ELISA, Microarray
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by dot blot, ELISA, and western blotting against both the immunizing peptide and His-containing recombinant proteins. CY3s are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 50μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).
: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).
: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).
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: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).
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- His Tag
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背景
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Synonyms: mouse anti-6X His Tag CY3 conjugated Antibody, CY3 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody, CY3, Cyanine 3
Background: 6X His Tag CY3 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
抗原
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