HERC5 抗体 (AA 793-1024)
Quick Overview for HERC5 抗体 (AA 793-1024) (ABIN6719405)
抗原
See all HERC5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 793-1024
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原理
- Anti-HERC5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-HERC5 Antibody Picoband® (ABIN6719405). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).
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亚型
- IgG
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anti-Hect Domain and RLD 5 (HERC5) (Middle Region) antibody
HERC5 适用: 人, Cow, 马, 兔, 大鼠, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (AA 153-284) antibodyHERC5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (AA 915-1024) antibodyHERC5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (AA 287-336) antibodyHERC5 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (AA 900-949) antibodyHERC5 适用: 人, Cow, 马, 兔, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (AA 360-700) antibodyHERC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (N-Term) antibodyHERC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Hect Domain and RLD 5 (HERC5) (N-Term) antibodyHERC5 适用: 人, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Hochrainer, K., Mayer, H., Baranyi, U., Binder, B. R., Lipp, J., Kroismayr, R. The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects. Genomics 85: 153-164, 2005. 2. Mitsui, K., Nakanishi, M., Ohtsuka, S., Norwood, T. H., Okabayashi, K., Miyamoto, C., Tanaka, K., Yoshimura, A., Ohtsubo, M. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins. Biochem. Biophys. Res. Commun. 266: 115-122, 1999. 3. Wong, J. J. Y., Pung, Y. F., Sze, N. S.-K., Chin, K.-C. HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets. Proc. Nat. Acad. Sci. 103: 10735-10740, 2006. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HERC5 (Hect Domain and RLD 5 (HERC5))
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别名
- HERC5
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背景
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Synonyms: E3 ISG15--protein ligase HERC5, Cyclin-E-binding protein 1, HECT domain and RCC1-like domain-containing protein 5, HERC5, CEB1, CEBP1
Tissue Specificity: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
Background: Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.
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分子量
- 117 kDa
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基因ID
- 51191
抗原
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