RPA1 抗体 (C-Term)
Quick Overview for RPA1 抗体 (C-Term) (ABIN6719369)
抗原
See all RPA1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 533-568, C-Term
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原理
- Anti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4)
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序列
- QESAEAILGQ NAAYLGELKD KNEQAFEEVF QNANFR
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) (ABIN6719369). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human RPA70, different from the related mouse sequence by three amino acids.
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亚型
- IgG2b
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anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (C-Term) antibodyRPA1 适用: 人 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 猴 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (Internal Region) antibodyRPA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) antibodyRPA1 适用: 人, 小鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibodyRPA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A1, 70kDa (RPA1) (AA 1-240) antibodyRPA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Erdile, L. F., Heyer, W.-D., Kolodner, R., Kelly, T. J. Characterization of a cDNA encoding the 70- kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication. J. Biol. Chem. 266: 12090-12098, 1991. Note: Erratum: J. Biol. Chem. 268: 2268 only, 1993. 2. Haring, S. J., Mason, A. C., Binz, S. K., Wold, M. S. Cellular functions of human RPA1: multiple roles of domains in replication, repair, and checkpoints. J. Biol. Chem. 283: 19095-19111, 2008. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- RPA1 (Replication Protein A1, 70kDa (RPA1))
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别名
- RPA1
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背景
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Synonyms: Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, Replication protein A 70 kDa DNA-binding subunit, N-terminally processed, RPA1, REPA1, RPA70
Tissue Specificity: Expressed in lung fibroblasts (at protein level). Expressed in monocytes. Highly expressed in liver, also found in kidney and brain.
Background: Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene. This gene is mapped to chromosome 17p13.3. Replication protein A (RPA) is a heterotrimeric single-strand DNA (ssDNA)-binding protein essential for DNA replication, repair, and recombination. It is composed of 70-kD (RPA1), 32-kD (RPA2), and 14-kD (RPA3) subunits. The RPA1 subunit is responsible for high-affinity ssDNA binding. The RPA complex was originally isolated as a factor essential for in vitro replication of the papovavirus SV40. It had been found that recombinant human RPA1, purified from bacteria, exhibited ssDNA-binding activity comparable to that of the complete RPA complex. RPA1 could substitute for the complete complex in stimulating the activity of DNA polymerase alpha-primase, but it could not substitute for the complete complex in SV40 DNA replication in vitro, suggesting an important functional role for the other subunits.
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分子量
- 12 kDa
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基因ID
- 6117
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UniProt
- P27694
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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