ERCC3 抗体 (AA 665-715)
Quick Overview for ERCC3 抗体 (AA 665-715) (ABIN6719310)
抗原
See all ERCC3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 665-715
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原理
- Anti-XPB/ERCC3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-XPB/ERCC3 Antibody Picoband® (ABIN6719310). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human XPB recombinant protein (Position: Q665-K715).
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亚型
- IgG
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anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (C-Term) antibody
ERCC3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (N-Term) antibodyERCC3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (AA 242-261) antibodyERCC3 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 3G4 unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (AA 513-782) antibodyERCC3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (AA 601-700) antibodyERCC3 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (AA 29-128) antibodyERCC3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (N-Term) antibodyERCC3 适用: 人, Cow, 犬, 兔, 斑马鱼, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (AA 512-782) antibodyERCC3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA Repair Protein Complementing XP-B Cells (ERCC3) (N-Term) antibodyERCC3 适用: 人, 大鼠, Cow, 犬, 兔, 斑马鱼, 豚鼠, Pig, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Coin, F., Oksenych, V., Egly, J.-M. Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair. Molec. Cell 26: 245-256, 2007. 2. Kim, T.-K., Ebright, R. H., Reinberg, D. Mechanism of ATP-dependent promoter melting by transcription factor IIH. Science 288: 1418-1421, 2000. 3. Le May, N., Dubaele, S., De Santis, L. P., Billecocq, A., Bouloy, M., Egly, J.-M.TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus. Cell 116: 541-550, 2004. -
说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))
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别名
- ERCC3
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背景
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Synonyms: General transcription and DNA repair factor IIH helicase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH 89 kDa subunit, TFIIH p89, Xeroderma pigmentosum group B-complementing protein, ERCC3, XPB, XPBC
Tissue Specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).
Background: XPB (xeroderma pigmentosum type B) is an ATP-dependent DNA helicase in humans that is a part of the TFIIH transcription factor complex. This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.
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分子量
- 89 kDa
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基因ID
- 2071
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UniProt
- P19447
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途径
- DNA Damage Repair
抗原
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