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NF-kB p65 抗体 (AA 51-100)

Name: NF-kB p65 抗体 (AA 51-100)
SKU: ABIN668961
Availability: OutOfStock

This anti-NF-kB p65 antibody is a 兔多克隆 antibody detecting NF-kB p65 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔和小鸡. This Primary Antibody has been cited in 30+ publications.

HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (NF-kB p65 抗体 (AA 51-100))

Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (ABIN668961), Unconjugated at 1:600 followed by conjugation to the secondary antibody and DAB staining (NF-kB p65 抗体 (AA 51-100))

IHC

Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated . (NF-kB p65 抗体 (AA 51-100))

FACS

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (NF-kB p65 抗体 (AA 51-100))

IHC (p)

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (NF-kB p65 抗体 (AA 51-100))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling in sodium citrate buffer (pH6. (NF-kB p65 抗体 (AA 51-100))

IHC (p)

Western blot analysis of P-Akt/Akt ratio, NF-kB and P-NF-kB proteins of rats fed either placebo, (A) B. (NF-kB p65 抗体 (AA 51-100))

7 images

(30 references)

产品编号 ABIN668961

发货至: 中国

Quick Overview for NF-kB p65 抗体 (AA 51-100) (ABIN668961)

抗原

See all NF-kB p65 (NFkBP65) 抗体

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

适用

人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡

宿主

兔

克隆类型

多克隆

标记

This NF-kB p65 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 51-100

交叉反应

小鸡, Cow, 人, 小鼠, 兔, 大鼠, 斑马鱼

预测反应

Dog,Cow,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human NFKBp65

亚型

IgG
anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (Center) antibody
NFkBP65 适用: 人, 小鼠 WB, IF, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 19-306) antibody
NFkBP65 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys314), (acLys315) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 50-180) antibody
NFkBP65 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人 WB, IHC, IP, IF 宿主: 小鼠 Monoclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Fontana, Plaza-Díaz, Robles-Bolívar, Valente-Godínez, Sáez-Lara, Abadía-Molina, Gómez-Llorented, Gil, Álvarez-Mercado et al.: "Bifidobacterium breve CNCM I-4035, Lactobacillus paracasei CNCM I-4034 and Lactobacillus rhamnosus CNCM I-4036 Modulate Macrophage Gene Expression and Ameliorate Damage Markers in the Liver of ..." in: Nutrients, Vol. 13, Issue 1, (2021) (PubMed).

Oyagbemi, Omobowale, Ola-Davies, Asenuga, Ajibade, Adejumobi, Afolabi, Ogunpolu, Falayi, Ayodeji, Hassan, Saba, Adedapo, Yakubu: "Ameliorative effect of Rutin on sodium fluoride-induced hypertension through modulation of Kim-1/NF-κB/Nrf2 signaling pathway in rats." in: Environmental toxicology, Vol. 33, Issue 12, pp. 1284-1297, (2019) (PubMed).

Xu, Liu, Yu, Wu, Lu: "Effect of recombinant Trichinella spiralis cysteine proteinase inhibitor on TNBS-induced experimental inflammatory bowel disease in mice." in: International immunopharmacology, Vol. 66, pp. 28-40, (2019) (PubMed).

Fattori, Borghi, Guazelli, Giroldo, Crespigio, Bussmann, Coelho-Silva, Ludwig, Mazzuco, Casagrande, Verri: "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." in: Pharmacological research, Vol. 120, pp. 10-22, (2018) (PubMed).

Sun, Nemoto, Hong, Sasaki: "Modulation of stromal cell-derived factor 1 alpha (SDF-1α) and its receptor CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation." in: BMC oral health, Vol. 17, Issue 1, pp. 26, (2018) (PubMed).

Xi, Wang, Chen, Yang, Hu, Zhang, Weng, Xu: "Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii)." in: European journal of histochemistry : EJH, Vol. 61, Issue 4, pp. 2834, (2018) (PubMed).

Lin, Geng, Zhao, Lin, Zhu, Tian: "MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 1, pp. 124-136, (2017) (PubMed).

Ganguly, Wen, Myer, Czech, Sahu, Steinmetz, Raman: "Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro." in: Nanoscale, Vol. 8, Issue 12, pp. 6542-54, (2016) (PubMed).

Yang, Zhong, Li, Xiong, Wang, Peng, Ye: "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 161-71, (2016) (PubMed).

Oyagbemi, Omobowale, Asenuga, Adejumobi, Ajibade, Ige, Ogunpolu, Adedapo, Yakubu: "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." in: Environmental toxicology, (2016) (PubMed).

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, (2016) (PubMed).

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, (2016) (PubMed).

Zhang, Wu, Deng, Zheng, Zhang, Deng, Chen, Ma, Wang, Yu, Kang, Wang: "High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5189-95, (2016) (PubMed).

Guo, Deng, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen: "Nickel chloride (NiCl2)-caused inflammatory responses via activation of NF-κB pathway and reduction of anti-inflammatory mediator expression in the kidney." in: Oncotarget, Vol. 6, Issue 30, pp. 28607-20, (2016) (PubMed).

Li, Cheng, Zhao, Guo, Liu, Zhang, Wang, Zhang, Pan, Nie: "ADAMTS-7 exhibits elevated expression in cartilage of osteonecrosis of femoral head and has a positive correlation with TNF- α and NF- κ B P65." in: Mediators of inflammation, Vol. 2015, pp. 196702, (2016) (PubMed).

Qian, Cai, Zhang, Wang, Qian, Hasegawa: "Effect of Daisaikoto on Expressions of SIRT1 and NF-kappaB of Diabetic Fatty Liver Rats Induced by High-Fat Diet and Streptozotocin." in: Yonago acta medica, Vol. 59, Issue 2, pp. 149-58, (2016) (PubMed).

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, (2015) (PubMed).

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, (2015) (PubMed).
抗原

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

别名

NFKB p65

背景

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA
Background: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

基因ID

5970

UniProt

Q04206

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins