CD45 抗体 (FITC)
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- 抗原 See all CD45 (PTPRC) 抗体
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 200-502-n85
- Supplier
- Rockland
- 原理
- CD45.1 Fluorescein Antibody
- 纯化方法
- Fluorescein conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.
- 过滤
- Sterile filtered
- 免疫原
- Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
- 克隆位点
- A20
- 亚型
- IgG2a kappa
- Labeling Ratio
- 2-8
- Top Product
- Discover our top product PTPRC Primary Antibody
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- 应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
Other: Sufficient to run approximately 500 tests
- 说明
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Anti-CD45.1 is tested for Flow Cytometry and useful for Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
- 有效期
- 6 months
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- 抗原
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- 别名
- Ptprc (PTPRC 产品)
- 背景
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Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Background: The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Gene Name: Ptprc
- UniProt
- P06800
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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