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CD45 抗体 (FITC)

PTPRC 适用: 小鼠 FACS, IHC, IP 宿主: 小鼠 Monoclonal A20 FITC
Rockland
产品编号 ABIN6657896
Supplier Product No.: 200-502-n85
发货至: 中国
  • 抗原 See all CD45 (PTPRC) 抗体
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    适用
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    • 221
    • 83
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    • 20
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    小鼠
    宿主
    • 295
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    小鼠
    克隆类型
    • 470
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    单克隆
    标记
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    This CD45 antibody is conjugated to FITC
    应用范围
    • 500
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    200-502-n85
    Supplier
    Rockland
    原理
    CD45.1 Fluorescein Antibody
    纯化方法
    Fluorescein conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.
    过滤
    Sterile filtered
    免疫原
    Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
    克隆位点
    A20
    亚型
    IgG2a kappa
    Labeling Ratio
    2-8
    Top Product
    Discover our top product PTPRC Primary Antibody
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    Other: Sufficient to run approximately 500 tests

    说明

    Anti-CD45.1 is tested for Flow Cytometry and useful for Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
    有效期
    6 months
  • 抗原
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    别名
    Ptprc (PTPRC 产品)
    背景

    Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

    Background: The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

    Gene Name: Ptprc

    UniProt
    P06800
    途径
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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