MCL-1 抗体 (Internal Region)
(9 references)-
- 抗原 See all MCL-1 (MCL1) 抗体
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
-
抗原表位
- Internal Region
-
适用
- 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MCL-1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity with Mcl-1 from other sources is unknown
- 纯化方法
- Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. .
- 免疫原
-
Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product MCL1 Primary Antibody
-
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 2-307) antibody
MCL1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Center) antibodyMCL1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibodyMCL1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IP 宿主: 兔 Monoclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 19-244) antibodyMCL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 120-220) antibodyMCL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibodyMCL1 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 8C6 unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 191-226) antibodyMCL1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 6-328) antibodyMCL1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: Rabbit Anti-Mcl-1 antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:10,000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
-
-
: "Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1." in: Cell death & disease, Vol. 11, Issue 6, pp. 442, (2021) (PubMed).
: "Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling." in: Cell death & disease, Vol. 9, Issue 5, pp. 470, (2018) (PubMed).
: "Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer." in: Cell reports, Vol. 24, Issue 13, pp. 3393-3403.e5, (2018) (PubMed).
: "JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins." in: Cell reports, Vol. 21, Issue 7, pp. 1910-1921, (2018) (PubMed).
: "Mcl-1 is a key regulator of the ovarian reserve." in: Cell death & disease, Vol. 6, pp. e1755, (2016) (PubMed).
: "The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland." in: Cell death and differentiation, Vol. 23, Issue 1, pp. 41-51, (2016) (PubMed).
: "Maritoclax induces apoptosis in acute myeloid leukemia cells with elevated Mcl-1 expression." in: Cancer biology & therapy, Vol. 15, Issue 8, pp. 1077-86, (2015) (PubMed).
: "Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1." in: Cell death & disease, Vol. 4, pp. e927, (2014) (PubMed).
: "Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration." in: Nature cell biology, Vol. 14, Issue 6, pp. 575-83, (2012) (PubMed).
-
: "Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1." in: Cell death & disease, Vol. 11, Issue 6, pp. 442, (2021) (PubMed).
-
- 抗原
- MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
- 别名
- Mcl-1 (MCL1 产品)
- 别名
- BCL2L3 antibody, EAT antibody, MCL1-ES antibody, MCL1L antibody, MCL1S antibody, Mcl-1 antibody, TM antibody, bcl2-L-3 antibody, mcl1/EAT antibody, AW556805 antibody, mcl-1 antibody, fb72f02 antibody, wu:fb70a08 antibody, wu:fb72f02 antibody, zfMLP1 antibody, zfMcl-1a antibody, zgc:109835 antibody, zgc:109763 antibody, E3 antibody, E3-2 antibody, RNE3 antibody, RNU107 antibody, SNORA63A antibody, elF-4AII antibody, MCL1, BCL2 family apoptosis regulator antibody, myeloid cell leukemia sequence 1 antibody, MCL1, BCL2 family apoptosis regulator a antibody, MCL1, BCL2 family apoptosis regulator b antibody, small nucleolar RNA, H/ACA box 63 antibody, MCL1 antibody, Mcl1 antibody, mcl1a antibody, mcl1b antibody, SNORA63 antibody
- 背景
-
Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.
Gene Name: Mcl1
- 基因ID
- 17210
- UniProt
- P97287
- 途径
- MAPK Pathway
-
