ERK1 抗体 (Internal Region)
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北京 101111
Quick Overview for ERK1 抗体 (Internal Region) (ABIN6657518)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-r20
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Supplier
- Rockland
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原理
- MAPK3 Antibody
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交叉反应 (详细)
- Anti-MAPK3 antibody is directed against human MAPK3 protein.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- MAPK3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human MAPK3.
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亚型
- IgG
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应用备注
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Immunohistochemistry_Dilution: 0.5-1 μg/mL
Western_Blot_Dilution: 0.1-0.5 μg/mL
Other: Immunocytochemistry: 0.5-1 μg/mL
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说明
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Anti-MAPK3 is tested for Immunohistochemistry -P, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.1 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide
Stabilizer: 5 mg BSA
Preservative: 0.05 mg Thimerosal -
储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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别名
- MAPK3
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背景
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Synonyms: ERT2,Extracellular signal-regulated kinase 1,
Background: Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis. This antibody is suitable for researchers interested in stem cell, neuroscience and cancer research.
Gene Name: MAPK3
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基因ID
- 5595
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NCBI登录号
- NP_001035145
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UniProt
- P27361
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
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