GFAP R416WT 抗体 (AA 411-422) (Alkaline Phosphatase (AP))
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- 抗原 See all GFAP R416WT products
- GFAP R416WT
- 抗原表位
- AA 411-422
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GFAP R416WT antibody is conjugated to Alkaline Phosphatase (AP)
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Anti-GFAP R416WT Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100% homology with the immunizing sequence. No cross-reactivity with other GFAP mutant proteins can be expected.
- 免疫原
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Immunogen: Anti-GFAP R416WT Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.
Immunogen Type: Peptide
- 克隆位点
- S206B-9
- 亚型
- IgG1
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-GFAP R416WT Alkaline Phosphatase Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- GFAP R416WT
- Abstract
- GFAP R416WT 产品
- 背景
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Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody
Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.
Gene Name: GFAP
- 基因ID
- 2670
- UniProt
- P14136
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