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Carboxylesterase 1G (CES1G) 抗体

CES1G 适用: 人 ELISA, IP, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6655641
发货至: 中国
  • 抗原
    Carboxylesterase 1G (CES1G)
    适用
    宿主
    克隆类型
    多克隆
    应用范围
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    纯化方法
    This product was prepared from monospecific antiserum by delipidation and defibrination.   The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie. IL-1 stimulated hCE1 production from fibroblasts).
    免疫原

    Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.

    Immunogen Type: Recombinant Protein

  • 应用备注

    Application Note: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user.   Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract.  In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Western Blot Dilution: 1:2,500 - 1:5,000

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • 抗原
    Carboxylesterase 1G (CES1G)
    别名
    Liver Carboxylesterase-1
    别名
    ACAT antibody, CE-1 antibody, CEH antibody, CES2 antibody, HMSE antibody, HMSE1 antibody, PCE-1 antibody, REH antibody, SES1 antibody, TGH antibody, hCE-1 antibody, Ces-1 antibody, Ces1 antibody, Ses-1 antibody, carboxylesterase 1 antibody, carboxylesterase 1G antibody, liver carboxylesterase antibody, similar to esterase 2 antibody, CES1 antibody, Ces1g antibody, LOC100009551 antibody, LOC684016 antibody
    背景

    Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH

    Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.

    Gene Name: CES1

    基因ID
    1066, 68508965
    UniProt
    P23141
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