CD14 抗体
(9 references)Quick Overview for CD14 抗体 (ABIN638430)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD14 Purified Low Endotoxin
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特异性
- The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
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交叉反应 （详细）
- Non-Human Primates, Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
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免疫原
- A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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亚型
- IgG1
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anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated
anti-CD14 (CD14) (AA 20-214) antibodyCD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated
anti-CD14 (CD14) (AA 20-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 14G1F3 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated
anti-CD14 (CD14) (AA 230-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated
anti-CD14 (CD14) (AA 54-83), (N-Term) antibodyCD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated
anti-CD14 (CD14) (AA 201-300) antibodyCD14 适用: 人, 小鼠, 大鼠, 绵羊, 犬, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2397 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人, Non-Human Primate FACS, WB, ELISA, IP 宿主: 小鼠 Monoclonal MEM-18 unconjugated
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应用备注
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ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100).
Functional application: The antibody MEM-18 completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
Flow cytometry: recommended dilution: 4 μg/mL.
Western blotting: Non-reducing conditions. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).
: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).
: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, (2005) (PubMed).
: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).
: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).
: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, (2001) (PubMed).
: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, (1989) (PubMed).
: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
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: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).
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- CD14
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别名
- CD14
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背景
- CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR
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基因ID
- 929
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UniProt
- P08571
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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